I actually have 4 tubes of M. ruginodis queens, all collected on the 21st of August. 3 of them have 3 queens each, and the other has 4. I chose to do a journal of the latter, because 4 queens seems more interesting than 3 queens.
Since collecting them, I have kept this "colony" confined to its test tube. I fed them in the tube as well as I could, which was difficult because the queens seemed to spend most of their time on the dry cotton bung. Today, I put the tube in a foraging arena and pulled out the bung. I dropped in a little apple flesh, and some bits of a defrosted Lasius niger queen that I found dead after the nuptial flights. Three of the queens promptly exited the tube and snuggled up next to it, two of them eventually doing some exploring. I saw one find the apple, but she gave it little attention before moving on, perhaps because I gave them all some sugary egg white last night. At this point, I'm slightly worried that most of the ants may have abandoned the interior of the test tube in favour of sitting next to it. Surely they'll eventually rejoin the remaining queen in their home eventually?