I'll be trying out my idea of wetting some small pieces of cotton wool to form a small wall and passage hole, and then pushing one further into the test tube to form a small chamber for the queen and brood.
And another for the test tube entrance. Wetting them to make it easy to mold the shape, and once they dry the shape will stick
I think the ants will like this.
And help prevent the panic I get with regular test tube setups when you drop in dead insects in or just outside the test tube.
Since the queen and brood is separated etc
And reduce air flow, make ants feel more secure and so on