More detail on your situation would be helpful. Given what you've provided, I hope this is beneficial for you.
If you're certain you're rearing C. sansabeanus, I can assume you're in the southwestern US. If you're in California, you may be in Argentine ant (Linepithema humile) territory. These small ants are very aggressive and can fit into tiny crevices, destroying enclosed colonies. I assume you're keeping your colony in the garage to maximize colony growth because it's warmer than the rest of the house, but garages are full of openings for other ants--and insects generally--to squeeze through. It may be better for you to keep the colony inside with an artificial heat source.
If you're dealing with an L. humile problem, they're also notorious for getting inside the home as well. I recommend using poison ant baits from your local hardware store to deal with them or any problematic ants you don't want getting inside. If your colonies' housing is secure, you won't have to worry about the ants you're keeping getting to it.
Regardless of the identity of your intruder, the act itself means it was an agressive species, and they likely killed your first workers. I've lost plenty of nanitics over the years for various reasons, and your queen may lay again. If I have to reset a queen for whatever reason, I've had pretty good success with these steps:
1. Put the queen in a small container. [I use 2.5 oz. plastic Solo brand soufflés cups with lids because I can buy them in bulk and I work with huge numbers of insects.]
2. Give her a cotton ball soaked with some sugar water. [If you want, give her some liquid protein as well.]
3. After 24-48 hours, set her up in a new test tube and treat her like a new queen. [After you put her in a new tube, it may help to chill her at hibernation temps for a week or two if she tugs at the cotton and seems stressed.]
If you're successful, keep in mind you really shouldn't be moving her until she has a sufficient number of workers and the test tube gets crowded or moldy. My Camponotus colonies usually stay in a test tube for two years before they're big enough to need an upgrade.
Good luck with everything!
Edited by Cameron C. Thomas, June 2 2017 - 6:42 AM.