6/23/17
When I woke up this morning I checked all of the tubes, and saw that 1 queen already had her first nanitic! Once I got home two more queens followed this pattern. So far, the queens each have 1-2 workers. As I am going out of state for the next 10 days, I have frozen some D. hydei (fruit flies) and placed 3 on a piece of tin foil for them to work on while I am gone. I'm also going to give them some cotton balls soaked in Sunburst to drink from (one queen is drinking from the cotton ball as I type this).
Bonus pic- Close up of pupae 2 days ago. You can see that some of them are close to eclosing as their mandibles have darkened. Before they eclose, the entire pupae will appear orange/yellow as opposed to white.