Lasius are stubborn to move. Keep that in mind in future formicariums, the ability to open it is needed.
With test tubes, use a long pair of tweezers to pull the cotton closer to the entrance where their nest scent if fainter, they tend to move quite readily then. All else fails, just put the cotton ball and ants all into the new outworld.
Dermy, in Nova Scotia he is like looking at Lasius neoniger, not Lasius niger as that is a European species (Occasionally found in the US as a hitch hiker).
When did you collect her?
When and for how long did they go through hibernation?
I am seeing a lot of condensation on the side of the test tube in the first picture, use a bit less cotton to plug the entrance so it dries out a little bit more.
I found putting a small piece of silicone tubing under hte cotton and letting the ants forage in a small setup seems to cause faster growth. Offer things like sugar water 24/7. I usually drop in live fruit flies to act as a living food source and my colonies take off quickly.