One of the queens is dead now!!!! The other is not harmed at all. Also, I now have a major!!!!!!!!!!!! I was shining my phone light on the test tube to see inside and out of the hole in the dirt where the queen and brood are, a major came out! I saw the major a couple days after the ants ate up a whole mealworm (large size, but I don't think that the meal worm was fully grown). The major has not come out of the test tube yet.
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