My estimate has been raised to 116, I undercounted quite a bit. There are probably more than 116, that's just an estimate. I have finished tubing them all up, and decided to test the polygyny of these, because in some populations they are polygynous. They have gotten along so far, and have laid a lot of eggs!
I have 4 duo queen tubes, all of which have a number of eggs. I took aside the one with the most to keep:
I also have 2 triple queen tubes, which were originally part of a group of 10 that I decided to split up.
And, I have 1 tube of 5 queens. The eggs are spread out because I had to transfer them little by little, but they are currently gathering them up:
All the rest are single queens. A lot of them laid a good amount of eggs, and I had about 10 deaths that I know of. This queen has one of the most amount of eggs of all the singles:
Unfortunately, I didn't get a shot of all 116 queens, but was able to lay out 70 to get this cool picture:
I'll update once they get larvae.
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Wishlist:
Atta sp (wish they were in CA), Crematogaster cerasi, Most Pheidole species