Honestly, I used to have the same worries as you. However, at this point in time, I have realized that most ants are not nearly as sensitive as I believed they would be when I first found my original queens.
I used to cover all my queens and was terrified to let them see light or even accidently, slightly bump one of their tubes. I have had a lot of far more experienced keepers than I assure me that, yes, although SOME ants might care SLIGHTLY, the vast majority don't. As long as you aren't picking up the tubes every day and shaking them or dropping them and such, the vast majority of your colonies will get used to light and vibrations.
My biggest colony, which happens to be Solenopsis invicta, scatter like wild at the slightest bump.. yet they are thriving.
My Camponotus castaneus, however, couldn't give the slightest of a care. Neither do my Pheidole bicarinata. I will say, half of my Dorymyrmex bureni queens care, the other half don't. My Brachymyrmex patagonicus do not seem to care at all, either.
Just be careful with them, but if you do handle them, it's not that big of a deal.
Again, that is for, in my experience, the majority of ants I have.. not all. You should definitely be most careful in the beginning stages of founding to kind of gauge which of your ants care and which don't.
Edited by Flu1d, June 3 2023 - 7:39 PM.