I have a bunch of semitestaceus in the same setup, a clear plastic box with a test tube. They are in my room which has a lot of lights. They've been thriving and have had a lot of nanitics, However one Queen a few weeks ago started leaving the test tube and kept walking around and grooming herself. I put her container in a dark closet but she still died.
Now this is happening with two more queens. For one I just put her in the test tube and closed it (so the colony is just in the test tube for now), for the other one I have added aluminum foil around the test tube so that it's dark, because maybe they are stressed out? This is strange because I have about 6 more colonies in the same place and those queens are not stressed or have any problems, And the queen behavior is real weird, I'd think as soon as I put them in the dark they would be okay and just go back in the test tube, but they don't. All of the nanitics are healthy and fine, and they usually stay in the tube tending to the eggs. I have not added any new food sources that could have pesticides or anything. Any ideas as to what is going on?