I think you may have misread the directness of my reply as something else. I'm not saying don't ask questions. I'm simply saying that you must not think for yourself until you have sufficient knowledge to make informed choices. Lots of members experiment before they know what they're doing, and end up unintentionally massacring perfectly capable ants in the process.
Solenopsis invicta is considered fully claustral. This means that the queens find shelter after mating, generally digging a hole in the ground, and rear their first brood entirely from their own fat reserves, never leaving the nest to forage for food. You are more likely to disturb and hinder the queen's progress by introducing food, not to mention the possible risk that member Myrmicinae observed.
As far as temperature, again, these ants usually dig a hole in the ground. Even when it is 100+ degrees outside, it is much cooler several inches below the soil. 90 F would be a maximum temperature for the speediest development—much higher and you risk stalling. I recommended 85F as a guideline—any lower than 80, and again, development will stall. An unairconditioned room that hovers around the mid 80s to low 90s max should be sufficient.
I looked again at the test tubes, and they are a lot larger in diameter than I would recommend, and with that also comes the fact that a lot of water probably seeped completely through the cotton. Did that happen, or is it dryish on the side where the ants are hugging? Larger test tubes can be tricky to seal properly, because the cotton must be packed tightly before stuffing into the test tube, which is where many users will fail.
Again, do ask questions. Nobody here will tell you off just for not knowing what you think you know, but they will always tell you when your ideas are likely to harm, rather than help, your objective of keeping ants.
Edited by drtrmiller, July 15 2015 - 6:50 PM.