To give a little context, lets rewind to march of this year.
I went to go check on my colonies I had in hibernation, and to my despair, found all of my colonies dead (it's confirmed, kept the testubes until late may and nothing changed in the ants). At first, I didn't know what could've caused this, the testubes had water and i fed them prior to putting them into hibernation. I felt distraught and had no motivation to continue keeping ants.
Several weeks later i wanted a diagnosis, i wanted to see what i did wrong. The first thing i did was inspect the tubes itself. One thing that stood out to me in the my formica colony's tube was the abundance of mold and fungi. Although this wasn't a smoking gun, as the testubes were left there for long enough for it to grow overtime, but it did give me some hope. And as for my other colonies, it is still a mystery. My guess is that is was a failed hibernation. The temperatures in my basement constantly fluctuate, which may stress the ants out to the point they die.
It wasn't all doom and gloom though, in that time from march until now, i have captured many queen ants that are flourishing. My myrmica rubra and camponotus novaeboracensis colonies are exploding in population and developing larvae.
As an antkeeper, i want to grow from this. So I ask you experienced ant keepers how to prevent things like this to happen again, and do a proper hibernation. It would be greatly appreciated.