Update 9-5-2016 Good news and bad news. I'll start with the bad and end with the good.
My large colony in the glass vase is pretty much dead now. Almost all their fungus has died, and most of the colony too.
I really don't know what the problem is. There is only one tiny spot left where the fungus is still growing, but it doesn't look healthy at all.
There are piles and piles of dead ants in the bottom chambers and some of them are starting to grow foreign fungus.
I took a chunk of donor fungus and put it in one of my plastic chambers with a water tank on top of the watering tube in the out world. I'm waiting to see if one of the workers will end up finding it. I'm curious to see what they will do, and if they move into it, I'm curious to see if the queen is even alive still.
As for the younger colony I was getting donor fungus from, their fungus started to die, and then not too long ago I found the queen dead.
Luckily the colony in the 8-chamber
formicarium that used to be my largest, is still recovering and still has some healthy fungus.
I was passing through a large desert wash about 20 miles west of Lake Havasu, Arizona two weeks ago, and found a few
Acromyrmex founding chambers from an earlier flight. I decided to stop there again that night on my way home to see if I might find a few of them out foraging. Sure enough, after finding a few and digging a few up, I brought home eight new queens. I gave each of these queens some donor fungus from the younger colony with the dying fungus just to see if there was really something wrong with the fungus, or if the problem could be more related to the colony itself.
A week later, some storms passed through that same area again, so I headed back out. I stayed the night out there and sure enough, the next morning around 9:00 AM,
Acromyrmex started flying. There were tons of them, so I came home with plenty queens this time. I gave them all small pieces of donor fungus from my one remaining healthy colony.
What's funny is this time about half of the queens spit out their fungus pellet. I figured I would wait and see if any of them would grow into a fungus garden, but just like before, they all got tangled up in the cotton of the test tube and eventually destroyed.