The Tetramorium colony had around 40 members or more when they all died off. It was very sudden. The Liometopum colony had around 100. They’ve lost half or more in around a day or so. When I watch them, it just seems like they slow down and curl up when I’m not looking. I haven’t seen any spasms.How large is a colony? Tetramorium sometimes have small die-offs, but if you feed them a lot they should have a steady supply of new workers. If the ants were freaking out and when they curled up and were obviously in agony, that might be a sign of nerve damage and toxins. A test you could do is storing some of the workers in a clean test tube setup and see how they react.
(then you'd know if something is wrong with the nest)
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