Jump to content

  • Chat
  •  
  •  

Welcome to Formiculture.com!

This is a website for anyone interested in Myrmecology and all aspects of finding, keeping, and studying ants. The site and forum are free to use. Register now to gain access to all of our features. Once registered and logged in, you will be able to create topics, post replies to existing threads, give reputation points to your fellow members, get your own private messenger, post status updates, manage your profile and so much more. If you already have an account, login here - otherwise create an account for free today!

Photo

Position Bias May Affect Results in Feeding Tests


  • Please log in to reply
4 replies to this topic

#1 Offline drtrmiller - Posted April 25 2020 - 11:51 AM

drtrmiller

    Vendor

  • Members
  • PipPipPip
  • 2,714 posts

Disclaimer: The following is an anecdotal account from multiple observations.  I did not set up the experiment with the intention of testing a hypothesis, but am simply reporting what I have seen after noticing the issue described.

 

 

Over the past 2 months, I have been offering unlimited amounts of Sunburst Ant Nectar to a wild colony of Linepithema humile that enjoys entering my house through small cracks.  Based on the amount they are drinking each day, with no discernable reduction in feeding activity over these past months, the colony appears to be very large.

 

Over the past 10 days or so, I filled up 5 byFormica Mega liquid feeders with 40 mL of Sunburst Ant Nectar each (200 mL total), and placed the feeders in a single row for the ants to drink.  The liquid content and the feeder design is the same for each, so the only variable is the position of the feeder.  Each feeder is almost touching the other--there is not much distance between them in the row.

 

After 10 days, I measured the net amount of liquid remaining in each feeder, by weight.  Here are the results, from left to right in the row of 5 feeders:

 

 

The ants are tending the feeders different amounts, despite the contents of each being identical.  In total, I would estimate the ants are consuming 10-15 mL of fluid per day.  The feeding activity appears to vary based on the temperature and environmental conditions outside, as noted by the density of ants along the foraging trail.

 

I conclude that the position/placement of the feeder is distorting the rate of feeding.  Therefore, in future food studies, it may be necessary to frequently randomize the placement of feeders or foods when conducting comparison studies of the attractiveness of closely related foods in order to eliminate the possibility of position bias.  How to do this in a way where the foraging trails are not also intermingled so as to affect the results, I have no idea.


Edited by drtrmiller, April 25 2020 - 11:56 AM.

  • nurbs, Ants_Dakota and Froggy like this


byFormica® is the manufacturer of the iconic nectar feeders and Sunburst Ant Nectar.
byFormica ant products always deliver consistent performance, convenience,
and reliability, making them among the most beloved ant foods and kit enjoyed by
ant keeping enthusiasts worldwide. For more information, visit www.byFormica.com.

#2 Offline VoidElecent - Posted April 25 2020 - 11:57 AM

VoidElecent

    Advanced Member

  • Members
  • PipPipPip
  • 1,339 posts
  • LocationPhiladelphia, PA.

I conclude that the position/placement of the feeder is distorting the rate of feeding. 

 

10-15 mL! Those are some thirsty ants! Out of curiosity, where are the feeders positioned relative to the nest entrance?



#3 Offline drtrmiller - Posted April 25 2020 - 12:05 PM

drtrmiller

    Vendor

  • Members
  • PipPipPip
  • 2,714 posts

 

I conclude that the position/placement of the feeder is distorting the rate of feeding. 

 

10-15 mL! Those are some thirsty ants! Out of curiosity, where are the feeders positioned relative to the nest entrance?

 

 

I have no idea where the nest is.  They're polydomal and relocate nests quite frequently. The foraging trail is about 10 feet long from the feeders to the crack in the wall where the ants are entering my house.  But I have no idea how far the foraging trail spans from that point to where queens and brood are nesting.  

 

As an aside, I had fun over the past few months moving the feeders farther and farther away to test if the ants would find a more efficient route entering through another area.  I've siliconed lots of little gaps in my drywall, which are common in bathrooms because of all the cutouts for showers, cabinets, and light switches, in order to reduce the points of entry.  But it's like whack-a-mole—every place I plug, they find a new way inside.


Edited by drtrmiller, April 25 2020 - 12:06 PM.

  • Ants_Dakota likes this


byFormica® is the manufacturer of the iconic nectar feeders and Sunburst Ant Nectar.
byFormica ant products always deliver consistent performance, convenience,
and reliability, making them among the most beloved ant foods and kit enjoyed by
ant keeping enthusiasts worldwide. For more information, visit www.byFormica.com.

#4 Offline BugFinder - Posted April 25 2020 - 12:09 PM

BugFinder

    Advanced Member

  • Members
  • PipPipPip
  • 872 posts
  • LocationSunnyvale, CA

 

I conclude that the position/placement of the feeder is distorting the rate of feeding. 

 

10-15 mL! Those are some thirsty ants! Out of curiosity, where are the feeders positioned relative to the nest entrance?

 

Like Terry said, probably a massive colony.  Lots of mouths to feed.


“If an ant carries an object a hundred times its weight, you can carry burdens many times your size.”  ― Matshona Dhliwayo

 

My Journals:

Pogonomyrmex subdentatus

Camponotus Vicinus

Camponotus sansabeanus

Tetramorium (sp)

Pogonomyrmex Californicus

My Ant Goals!


#5 Offline Serafine - Posted May 17 2020 - 3:19 AM

Serafine

    Advanced Member

  • Members
  • PipPipPip
  • 1,812 posts
  • LocationGermany

This may not even be a coherent thing but caused by more-or-less random chance - basically the feeders that get the most workers initially may get a stronger mark than the other ones and thus attract more workers.

The terrain surrounding the feeders and the layout of their trail may affect it further, as well the way the ants fan out when they hit the blob of ants surrounding the closest feeder (if the ants do in fact act like little robots and fan out for a rather consistent distance this could explain feeder #3 being mostly omitted (because of the blob in front of feeder #1 and #2) but then again it could just be random chance or any other factor).

 

I've observed positional bias even in my comparable small 60x30cm outworld, the feeders that are emptied the quickest seem to be the ones close to nest entrances and highly frequented areas (trails, crossroads).


Edited by Serafine, May 17 2020 - 4:15 AM.

  • drtrmiller and Nathant2131 like this

We should respect all forms of consciousness. The body is just a vessel, a mere hull.

Welcome to Lazy Tube - My Camponotus Journal





1 user(s) are reading this topic

0 members, 1 guests, 0 anonymous users