Hi everyone, I caught 4 Prenolepis Imparis queens yesterday and have now put them in test tube setups. I've read the care sheets and journals on this species, and they are also my first queens. I've got some questions on how I should care for them. They are in a dark box on a shelf and my house temp is set for ~68 degrees. I currently have them half on a heating mat (in an attempt to get a gradient).
1. I've read that you should combine queens for better success rate? Would I want to do 2 and 2 or 3 and 1 or put all 4 together. Or should I just leave them in their own test tubes?
2. Should I use a heating mat? I've read that ants grow faster with heat, but this species specifically don't like heating.
Pictures will hopefully come tonight.