Thx for the feedback!
The racks will be kept inside a closet, so they will be mostly dark anyways. I will mostly only disturb them for monitoring, food, water etc. The racks being too big and smaller racks causing less disturbance is a good remark that might be very true. If it seems that the ants will be disturbed too much, I might reduce the rack size. If it doesn't, I'll enjoy watching many queen ants caring for their first broods.
I love the part where the queens are raising their first workers. I'm pretty sure it will be interesting for me. Being able to provide others with colonies that are past their first vulnerable period is also something that I would probably enjoy a lot. I'm mostly worried about too many colonies outgrowing their test tube and having to care for them with nests etc. which might require quite more time, but I'll have to get a feeling on how much work it'll require.
@rptraut: The debate on wether red light works or not is a thing I'm following very carefully.
I am not surprised that lasius species may still be restless. At least the red acrylic dims the light somewhat. It also makes it easier to monitor when I open up the closet without disturbing them too much. If it doesn't work well enough, I'll probably build a thin wooden cover that I can just put over the racks. If it works at first in full darkness, I might move on to putting one of the racks out in the open with just a few filled testtubes to see what happens.